Journal: International Journal of Molecular Sciences

Article Title: Umbilical Cord Mesenchymal Stromal Cell-Derived Exosomes Rescue the Loss of Outer Hair Cells and Repair Cochlear Damage in Cisplatin-Injected Mice

doi: 10.3390/ijms22136664

Figure Lengend Snippet: Effects of intraperitoneal injection of umbilical cord mesenchymal stromal cell (UCMSC) exosomes on cisplatin-induced hearing loss in mice. ( A ) Schematic of the experimental design of chronic administration of cisplatin in mice with induced hearing loss. Cisplatin (4 mg/kg) was injected intraperitoneally once per day for 5 consecutive days. UCMSC exosomes (1.2 μg/μL) in 100 μL of medium were administered intraperitoneally the same day after cisplatin injection. After 7 days, the hearing thresholds of all groups of mice were measured using an auditory brain response (ABR) instrument. Next, the mice were sacrificed to collect cochlear tissue for immunohistochemical staining. ( B ) ABR measurements revealed that UCMSC-exosome treatment significantly reduced the hearing thresholds at 8 and 12 kHz in the cisplatin-injected mice n = 4–8 per group. ( C ) The representative results of ABR at 12 kHz in each group of mice are shown. ( D ) Cisplatin-injected mice had significant loss of OHCs at the middle and basal regions compared with the control group. After UCMSC-exosome treatment in cisplatin-injected mice, the loss of OHCs at the middle and basal regions was significantly reduced compared with cisplatin-injected mice, n = 2–6 per group. Statistical significance was defined as p < 0.05.

Article Snippet: For both injection routes, all mice were euthanized 1 week after injection to measure their auditory brainstem response (ABR) (Biopac Systems, Inc., Goleta, CA, United States).

Techniques: Injection, Immunohistochemical staining, Staining, Control

Journal: International Journal of Molecular Sciences

Article Title: Umbilical Cord Mesenchymal Stromal Cell-Derived Exosomes Rescue the Loss of Outer Hair Cells and Repair Cochlear Damage in Cisplatin-Injected Mice

doi: 10.3390/ijms22136664

Figure Lengend Snippet: RNA-seq analyses for evaluating the effects of umbilical cord mesenchymal stromal cell (UCMSC) exosomes on the mouse miRNA expression patterns of the cochlear tissues of mice with cisplatin-induced hearing loss. Cochlear tissues were collected from a representative mouse in each group of mice according to the results for auditory brain response (ABR). Total RNA was extracted from the cochlear tissues and analyzed with RNA-seq. Notably, the treatment response of miRNAs (mmu-miR125b-5p, mmu-miR125a-5p, and mmu-miR127-3p) in inner ear tissues after UCMSC-exosome treatment overlapped with that of miRNAs present in UCMSC exosomes, including human miRNAs (125b-5p, 125a-5p, and 127-3p). This may be related to the direct uptake of homologous miRNAs of UCMSC exosomes by inner ear cells or the indirect induction of murine homology of miRNAs.

Article Snippet: For both injection routes, all mice were euthanized 1 week after injection to measure their auditory brainstem response (ABR) (Biopac Systems, Inc., Goleta, CA, United States).

Techniques: RNA Sequencing, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Umbilical Cord Mesenchymal Stromal Cell-Derived Exosomes Rescue the Loss of Outer Hair Cells and Repair Cochlear Damage in Cisplatin-Injected Mice

doi: 10.3390/ijms22136664

Figure Lengend Snippet: Effects of round window injection of umbilical cord mesenchymal stromal cell (UCMSC) exosomes on cisplatin-induced hearing loss in mice. ( A ) Schematic of the experimental design of chronic cisplatin administration for inducing hearing loss in mice. Cisplatin (4 mg/kg) was injected intraperitoneally once per day for 5 consecutive days, and 3 days later, UCMSC exosomes (1.2 mg/mL) in 10 μL of medium were administered through round window injection. After 7 days, the hearing thresholds of all groups of mice were measured using an auditory brain response (ABR) instrument at 12 kHz. Next, the mice were killed to collect cochlear tissue for immunohistochemical staining. ( B ) The results of ABR measurements revealed that cisplatin-injected mice had significantly impaired hearing function at 12 kHz compared with the control group. After UCMSC-exosome treatment in the cisplatin-injected mice, the hearing threshold at 12 kHz was significantly reduced compared with that of the cisplatin-injected mice, n = 6–14 per group. ( C ) Representative results of ABR at 12 kHz in each group of mice are shown. Statistical significance was defined as p < 0.05.

Article Snippet: For both injection routes, all mice were euthanized 1 week after injection to measure their auditory brainstem response (ABR) (Biopac Systems, Inc., Goleta, CA, United States).

Techniques: Injection, Immunohistochemical staining, Staining, Control

Journal: Aging (Albany NY)

Article Title: N-acetyl-cysteine prevents age-related hearing loss and the progressive loss of inner hair cells in γ-glutamyl transferase 1 deficient mice

doi: 10.18632/aging.100927

Figure Lengend Snippet: Mean (+/−SEM) auditory brainstem response (ABR) thresholds of +/+, +/dwg and dwg/dwg mice at 1, 3, 6 and 9 months of age at ( A ) 8 kHz, ( B ) 16 kHz and ( C ) 32 kHz. ( D ) Mean (+/−SEM) ABR threshold shifts of +/dwg and dwg/dwg mice relative to +/+ mice at 1, 3, 6 and 9 months of age at 8, 16 and 32 kHz (n= 29, 29, 26 and 15 for dwg/dwg mice at 1, 3, 6 and 9 months of age; n = 11, 12, 12, 12 for +/+ mice at 1, 3, 6 and 9 months of age and n = 12, 12, 12 and 12 for +/dwg mice at 1, 3, 6 and 9 months of ages).

Article Snippet: Breeding, genotyping and auditory brainstem response (ABR) hearing tests were conducted at the Jackson Laboratory and the cochleae from these mice were fixed and shipped to the University at Buffalo for histological evaluation.

Techniques: